Generator

Part:BBa_K152005

Designed by: David Johnston Monje   Group: iGEM08_Guelph   (2008-10-30)


Promoterless Synthetic Operon of CrtE,B,I,Y and GFP

This operon provides the full set of bacterial open reading frames necessary for beta carotene production with a GFP and termination stop signals tacked on at the end for good measure! crtE,B,I,Y coding regions from Erwinia uredovora each with a rbs preceeding the open reading frame. Likewise, the RBS-GFP-Term from biobrick BBa_E0240 is included on the end to provide a visual signal affirming transcription of preceeding 4 genes. There is no promoter on this construct, and after this GFP biobrick, no more genes can be added.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1941
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1478
    Illegal NgoMIV site found at 1608
    Illegal AgeI site found at 732
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5199


[edit]
Categories
//function/biosynthesis/isoprenoid
Parameters
None